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Locum Tenens Pathology Job in Locums Pathology coverage needed in central Mississippi Mississippi with Weatherby Locums
Job 9554776-0002 Established practice in MS is looking for a pathologist for a few days of vacation coverage. AP/CP board certification or recent board eligibility is a must. Locums physician will sign
Locum Tenens Pathology Job in Community hospital in scenic MD has locums-to-perm Pathology opportunity Maryland with Weatherby Locums
Job 9571215-0005 Locums opportunity available at Maryland community hospital. Close proximity to Baltimore and Washington, DC means easy access to big-city amenities. Board certified pathologist needed,
Locum Tenens Pathology Job in Locums position available in WA for board-certified Pathologist Washington with Weatherby Locums
Job 9558851-0012 Pathology board-certified physician needed for two weeks a month in Seattle, Washington area free-standing lab setting. Private lab facility does not require hospital privileging, but

Annals of Clinical Microbiology and Antimicrobials - Latest articles

Brachyspira pilosicoli bloodstream infections: Case report and review of the literature
Lilia Bait-Merabet, Arnaud Thille, Patrick Legrand, Christian Brun-Buisson and Vincent Cattoir Thu, 25 Sep 2008 00:00:00 -0000
Brachyspira pilosicoli is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. Here, we describe the case of a B. pilosicoli spirochetemia in a 53-year-old male patient suffering from cardiogenic shock. This fastidious bacterium was isolated from blood, likely after translocation from the intestinal tract. Blood cultures were positive after 5 days of incubation (one day after the patient's death), highlighting the problem of the recovery of such type of fastidious bacterium. Identification was achieved by molecular methods (16S rRNA sequencing). A review of the English literature found only 8 cases of bacteremia caused by B. pilosicoli, mostly in immunocompromised or critically ill patients. Finally, difficulties in rapid and accurate diagnosis of B. pilosicoli bloodstream infections, in vitro antimicrobial susceptibility of human clinical isolates, and therapeutic options are discussed.
Predicting the sensitivity and specificity of published real-time PCR assays
Gordon H Lemmon and Shea N Gardner Thu, 25 Sep 2008 00:00:00 -0000
Background: In recent years real-time PCR has become a leading technique for nucleic acid detection and quantification. These assays have the potential to greatly enhance efficiency in the clinical laboratory. Choice of primer and probe sequences is critical for accurate diagnosis in the clinic, yet current primer/probe signature design strategies are limited, and signature evaluation methods are lacking. Methods: We assessed the quality of a signature by predicting the number of true positive, false positive and false negative hits against all available public sequence data. We found real-time PCR signatures described in recent literature and used a BLAST search based approach to collect all hits to the primer-probe combinations that should be amplified by real-time PCR chemistry. We then compared our hits with the sequences in the NCBI taxonomy tree that the signature was designed to detect. Results: We found that many published signatures have high specificity (almost no false positives) but low sensitivity (high false negative rate). Where high sensitivity is needed, we offer a revised methodology for signature design which may designate that multiple signatures are required to detect all sequenced strains. We use this methodology to produce new signatures that are predicted to have higher sensitivity and specificity. Conclusions: We show that current methods for real-time PCR assay design have unacceptably low sensitivities for most clinical applications. Additionally, as new sequence data becomes available, old assays must be reassessed and redesigned. A standard protocol for both generating and assessing the quality of these assays is therefore of great value. Real-time PCR has the capacity to greatly improve clinical diagnostics. The improved assay design and evaluation methods presented herein will expedite adoption of this technique in the clinical lab.
Antimicrobial activity of some sulfonamide derivatives on clinical isolates of Staphylococus aureus
Yeliz Genç, Reşit Özkanca and Yunus Bekdemir Wed, 20 Aug 2008 00:00:00 -0000
Background: Staphylococcus aureus is a non-motile, gram positive, non-sporforming, facultative anaerobic microorganism. It is one of the important bacteria as a potential pathogen specifically for nosocomial infections. The sulfonamide derivative medicines are preferred to cure infection caused by S. aureus due to methicillin resistance. Methods: Antimicrobial activity of four sulfonamide derivatives have been investigated against 50 clinical isolates of S. aureus and tested by using MIC and disc diffusion methods. 50 clinical isolate which collected from specimens of patients who are given medical treatment in Ondokuz Mayis University Medical School Hospital. A control strain of S. aureus ATCC 29213 was also tested. Results: The strongest inhibition was observed in the cases of I [N-(2-hydroxy-4-nitro-phenyl)-4-methyl-benzensulfonamid], and II [N-(2-hydroxy-5-nitro-phenyl)-4-methyl-benzensulfonamid] against S. aureus. Compound I [N-(2-hydroxy-4-nitro-phenyl)-4-methyl-benzensulfonamid] showed higher effect on 21 S. aureus MRSAisolates than oxacillin antibiotic. Introducing an electron withdrawing on the ring increased the antimicrobial activity remarkably. Conclusion: This study may help to suggest an alternative possible leading compound for development of new antimicrobial agents against MRSA and MSSA resistant S. aureus. It was also shown here that that clinical isolates of 50 S. aureus have various resistance patterns against to four sulfonamide derivatives. It may also be emphasized here that in vitro antimicrobial susceptibility testing results for S. aureus need standardization with further studies and it should also have a correlation with in vivo therapeutic response experiments.

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