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Seeking Skilled Pathologist for Medical Director Position in Midwest :: Minnesota :: CompHealth Inc
Job 9110540 A large, well established Pathology Group in the Midwest is seeking an experienced Pathologist to serve as director. If you are an excellent communicator and educator, with leadership background
Fellowship Trained Dermatopathologist needed in Sunny Metro Arizona :: Arizona :: CompHealth Inc
Job 919845 Fellowship Trained Dermatopathologist needed in Sunny Metro Arizona Fellowship Trained Dermatopathologist urgently needed for a growing business Serve two area hospitals Board certification
Facility in Pennsylvania has excellent opportunity for Pathologist :: Pennsylvania :: Weatherby Locums
Job 9598516-0001 CLIA certified lab seeks locums physician to cover full time staff. Dates needed are about a few days up to a week for vacation coverage in the summer or fall. Surgical Pathology,
Annals of Clinical Microbiology and Antimicrobials - Latest Articles
Activity of tannins from Stryphnodendron adstringens on Cryptococcus neoformans: effects on growth, capsule size and pigmentation
Kelly IshidaSonia RozentalJoao de MelloCelso Nakamura Thu, 05 Nov 2009 00:00:00 -0000
Background: Stryphnodendron adstringens (Mart.) Coville, Leguminosae, also known in Brazil as barbatimao, is rich in tannins and many flavan-3-ols and proanthocyanidins such as prodelphinidins and prorobinetinidins. Previous studies have demonstrated several pharmacological properties of tannins from barbatimao, including anti-candidal activity. Methods: The antifungal activity of proanthocyanidin polymeric tannins from Stryphnodendron adstringens (subfraction F2.4) was evaluated against three strains of Cryptococcus neoformans with different capsule expressions, using the broth microdilution technique, light microscopy and transmission electron microscopy. The effect of subfraction F2.4 on C. neoformans and melanoma mammalian cells pigmentation was also evaluated. Results: Although susceptibility assays revealed MIC values quite similar (between 2.5 and 5.0 ug/ml), analyses of MFC values revealing that the acapsular mutant Cap 67 was more susceptible to be killed by the subfraction F2.4 (MFC = 20 ug/ml) than the two tested capsular strains (T1-444 and ATCC 28957) (MFC > 160 ug/ml). Optical and electron microscopy experiments revealed relevant alterations in cell shape and size in all strains treated with 1 and 2.5 ug/ml of subfraction F2.4. Capsule size of the capsular strains decreased drastically after subfraction F2.4 treatment. In addition, ultrastructural alterations such as cell wall disruption, cytoplasm extraction, mitochondria swelling, increase in the number of cytoplasmic vacuoles and formation of membranous structures in the cytoplasm were also observed in treated yeasts. Incubation with subfraction F2.4 also decreased C. neoformans pigmentation, however, did not interfere in melanization of B16F10 mammalian cells. Conclusion: Our data indicate that tannins extracted from S. adstringens interfered with growth, capsule size and pigmentation, all important virulence factors of C. neoformans, and may be considered as a putative candidate for the development of new antifungal agents.
Application of a high throughput Alamar blue biofilm susceptibility assay to Staphylococcus aureus biofilms
Robin PettitChristine WeberGeorge Pettit Tue, 27 Oct 2009 00:00:00 -0000
Background: Staphylococcus aureus and S. epidermidis biofilms differ in structure, growth and regulation, and thus the high-throughput method of evaluating biofilm susceptibility that has been published for S. epidermidis cannot be applied to S. aureus without first evaluating the assay's reproducibility and reliability with S. aureus biofilms. Methods: Staphylococcus aureus biofilms were treated with eleven approved antibiotics, lysostaphin, or ConfliktĀ®, exposed to the oxidation reduction indicator Alamar blue, and reduction relative to untreated controls was determined visually and spectrophotometrically. The minimum biofilm inhibitory concentration (MBIC) was defined as ā¤ 50% Alamar blue reduction and a purple/blue well 60 min after the addition of Alamar blue. Because all of the approved antibiotics had MBICs >128 Ī¼g/ml (most >2048 Ī¼g/ml), lysostaphin and ConfliktĀ®, with relatively low MBICs, were used to correlate Alamar blue reduction with 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction and viable counts (CFU/ml) for S. aureus ATCC 29213 and three clinical isolates. Alamar blue's stability and lack of toxicity allowed CFU/ml to be determined from the same wells as Alamar blue absorbances. Results: Overall, Alamar blue reduction had excellent correlation with XTT reduction and with CFU/ml. For ATCC 29213 and two clinical isolates treated with lysostaphin or ConfliktĀ®, Alamar blue reduction had excellent correlation with XTT reduction (r = 0.93-0.99) and with CFU/ml (r = 0.92-0.98). For one of the clinical isolates, the results were moderately correlated for ConfliktĀ® (r = 0.76, Alamar blue vs. XTT; r = 0.81, Alamar blue vs. CFU/ml) and had excellent correlation for lysostaphin (r = 0.95, Alamar blue vs. XTT; r = 0.97, Alamar blue vs. CFU/ml). Conclusion: A reliable, reproducible method for evaluating biofilm susceptibility was successfully applied to S. aureus biofilms. The described method provides researchers with a simple, nontoxic, relatively inexpensive, high throughput measure of viability after drug treatment. A standardized biofilm Alamar blue assay should greatly increase the rate of discovery of S. aureus biofilm specific agents.
Empiric antibiotic therapy in acute uncomplicated urinary tract infections and fluoroquinolone resistance: a prospective observational study
Cenk AypakAdalet AltunsoyNursen Duzgun Sat, 24 Oct 2009 00:00:00 -0000
Background: The aims of this study were to determine the antimicrobial susceptibility patterns of urinary isolates from community acquired acute uncomplicated urinary tract infections (uUTI) and to evaluate which antibiotics were empirically prescribed in the outpatient management of uUTI. Methods: Among the patients which were admitted to outpatient clinics of Ankara University Medical Faculty, Ibni-Sina Hospital during 2005-2006, a total of 429 women between the age of 18 and 65 years old who were clinically diagnosed with uUTI and to whom prescribed empirical antibiotics were enrolled in this prospective observational study. Patients' demographical data, urine culture results, resistance rates to antimicrobial agents and prescribed empiric antimicrobial therapy were analyzed. Results: Totally 390 (90.9%) patients among all study population were requested for urine culture by their physicians. 150 (38.5%) of these urine cultures were positive. The most common isolated uropathogen was Escherichia coli (E. coli) (71.3%). The variations of uropathogens according to age and menopause status were not significantly different.The resistance rates of E. coli isolates for ampicillin, ampicillin-sulbactam, amoxicillin-clavulonate, cefuroxime, ceftriaxone, fluoroquinolones (FQ), co-trimoxazole (TMP-SMX) and gentamicin were 55.1%, 32.7%, 32.7%, 23.4%, 15.9%, 25.2%, 41.1%, 6.1% respectively. FQ were the most common prescribed antibiotics (77.9%) (P < 0.001), followed by TMP-SMX (10.7%), fosfomycin (9.2%), nitrofurantoin (2.1%). Treatment durations were statistically longer than the recommended 3-day course (P < 0.001). Conclusion: Empirical use of FQ in uUTI should be discouraged because of increased antimicrobial resistance rates.
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Seeking Skilled Pathologist for Medical Director Position in Midwest :: Minnesota :: CompHealth Inc
Job 9110540 A large, well established Pathology Group in the Midwest is seeking an experienced Pathologist to serve as director. If you are an excellent communicator and educator, with leadership background
Fellowship Trained Dermatopathologist needed in Sunny Metro Arizona :: Arizona :: CompHealth Inc
Job 919845 Fellowship Trained Dermatopathologist needed in Sunny Metro Arizona Fellowship Trained Dermatopathologist urgently needed for a growing business Serve two area hospitals Board certification
Facility in Pennsylvania has excellent opportunity for Pathologist :: Pennsylvania :: Weatherby Locums
Job 9598516-0001 CLIA certified lab seeks locums physician to cover full time staff. Dates needed are about a few days up to a week for vacation coverage in the summer or fall. Surgical Pathology,
Annals of Clinical Microbiology and Antimicrobials - Latest Articles
Activity of tannins from Stryphnodendron adstringens on Cryptococcus neoformans: effects on growth, capsule size and pigmentation
Kelly IshidaSonia RozentalJoao de MelloCelso Nakamura Thu, 05 Nov 2009 00:00:00 -0000
Background: Stryphnodendron adstringens (Mart.) Coville, Leguminosae, also known in Brazil as barbatimao, is rich in tannins and many flavan-3-ols and proanthocyanidins such as prodelphinidins and prorobinetinidins. Previous studies have demonstrated several pharmacological properties of tannins from barbatimao, including anti-candidal activity. Methods: The antifungal activity of proanthocyanidin polymeric tannins from Stryphnodendron adstringens (subfraction F2.4) was evaluated against three strains of Cryptococcus neoformans with different capsule expressions, using the broth microdilution technique, light microscopy and transmission electron microscopy. The effect of subfraction F2.4 on C. neoformans and melanoma mammalian cells pigmentation was also evaluated. Results: Although susceptibility assays revealed MIC values quite similar (between 2.5 and 5.0 ug/ml), analyses of MFC values revealing that the acapsular mutant Cap 67 was more susceptible to be killed by the subfraction F2.4 (MFC = 20 ug/ml) than the two tested capsular strains (T1-444 and ATCC 28957) (MFC > 160 ug/ml). Optical and electron microscopy experiments revealed relevant alterations in cell shape and size in all strains treated with 1 and 2.5 ug/ml of subfraction F2.4. Capsule size of the capsular strains decreased drastically after subfraction F2.4 treatment. In addition, ultrastructural alterations such as cell wall disruption, cytoplasm extraction, mitochondria swelling, increase in the number of cytoplasmic vacuoles and formation of membranous structures in the cytoplasm were also observed in treated yeasts. Incubation with subfraction F2.4 also decreased C. neoformans pigmentation, however, did not interfere in melanization of B16F10 mammalian cells. Conclusion: Our data indicate that tannins extracted from S. adstringens interfered with growth, capsule size and pigmentation, all important virulence factors of C. neoformans, and may be considered as a putative candidate for the development of new antifungal agents.
Application of a high throughput Alamar blue biofilm susceptibility assay to Staphylococcus aureus biofilms
Robin PettitChristine WeberGeorge Pettit Tue, 27 Oct 2009 00:00:00 -0000
Background: Staphylococcus aureus and S. epidermidis biofilms differ in structure, growth and regulation, and thus the high-throughput method of evaluating biofilm susceptibility that has been published for S. epidermidis cannot be applied to S. aureus without first evaluating the assay's reproducibility and reliability with S. aureus biofilms. Methods: Staphylococcus aureus biofilms were treated with eleven approved antibiotics, lysostaphin, or ConfliktĀ®, exposed to the oxidation reduction indicator Alamar blue, and reduction relative to untreated controls was determined visually and spectrophotometrically. The minimum biofilm inhibitory concentration (MBIC) was defined as ā¤ 50% Alamar blue reduction and a purple/blue well 60 min after the addition of Alamar blue. Because all of the approved antibiotics had MBICs >128 Ī¼g/ml (most >2048 Ī¼g/ml), lysostaphin and ConfliktĀ®, with relatively low MBICs, were used to correlate Alamar blue reduction with 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction and viable counts (CFU/ml) for S. aureus ATCC 29213 and three clinical isolates. Alamar blue's stability and lack of toxicity allowed CFU/ml to be determined from the same wells as Alamar blue absorbances. Results: Overall, Alamar blue reduction had excellent correlation with XTT reduction and with CFU/ml. For ATCC 29213 and two clinical isolates treated with lysostaphin or ConfliktĀ®, Alamar blue reduction had excellent correlation with XTT reduction (r = 0.93-0.99) and with CFU/ml (r = 0.92-0.98). For one of the clinical isolates, the results were moderately correlated for ConfliktĀ® (r = 0.76, Alamar blue vs. XTT; r = 0.81, Alamar blue vs. CFU/ml) and had excellent correlation for lysostaphin (r = 0.95, Alamar blue vs. XTT; r = 0.97, Alamar blue vs. CFU/ml). Conclusion: A reliable, reproducible method for evaluating biofilm susceptibility was successfully applied to S. aureus biofilms. The described method provides researchers with a simple, nontoxic, relatively inexpensive, high throughput measure of viability after drug treatment. A standardized biofilm Alamar blue assay should greatly increase the rate of discovery of S. aureus biofilm specific agents.
Empiric antibiotic therapy in acute uncomplicated urinary tract infections and fluoroquinolone resistance: a prospective observational study
Cenk AypakAdalet AltunsoyNursen Duzgun Sat, 24 Oct 2009 00:00:00 -0000
Background: The aims of this study were to determine the antimicrobial susceptibility patterns of urinary isolates from community acquired acute uncomplicated urinary tract infections (uUTI) and to evaluate which antibiotics were empirically prescribed in the outpatient management of uUTI. Methods: Among the patients which were admitted to outpatient clinics of Ankara University Medical Faculty, Ibni-Sina Hospital during 2005-2006, a total of 429 women between the age of 18 and 65 years old who were clinically diagnosed with uUTI and to whom prescribed empirical antibiotics were enrolled in this prospective observational study. Patients' demographical data, urine culture results, resistance rates to antimicrobial agents and prescribed empiric antimicrobial therapy were analyzed. Results: Totally 390 (90.9%) patients among all study population were requested for urine culture by their physicians. 150 (38.5%) of these urine cultures were positive. The most common isolated uropathogen was Escherichia coli (E. coli) (71.3%). The variations of uropathogens according to age and menopause status were not significantly different.The resistance rates of E. coli isolates for ampicillin, ampicillin-sulbactam, amoxicillin-clavulonate, cefuroxime, ceftriaxone, fluoroquinolones (FQ), co-trimoxazole (TMP-SMX) and gentamicin were 55.1%, 32.7%, 32.7%, 23.4%, 15.9%, 25.2%, 41.1%, 6.1% respectively. FQ were the most common prescribed antibiotics (77.9%) (P < 0.001), followed by TMP-SMX (10.7%), fosfomycin (9.2%), nitrofurantoin (2.1%). Treatment durations were statistically longer than the recommended 3-day course (P < 0.001). Conclusion: Empirical use of FQ in uUTI should be discouraged because of increased antimicrobial resistance rates.
Sites:
What is Pathology ?: A consumer guide, including how to understand a pathology report and the process undertaken to reach a diagnosis.Armed Forces Institute of Pathology: An agency of the United States Department of Defense offering consultation, education and research. Details of services and resources, and a list of publications.
Atlas of Gastrointestinal Endoscopy: Images of diseased tissue throughout the tract, including tumours, ulcers and inflammatory bowel disease.
Atlas of Neuro-Oncology: ANOCEF's Atlas of Neuro-Oncology
Fibroblast Biology and Pathology at BioBitField: Information on research into the causes and treatment of fibrosis and scleroderma.
Indiana University : Pathology Education Resources: PerlJam is the product of the Pathology Education Resources Laboratory of the Department of Pathology at Indiana University School of Medicine. The site is intended to provide instructional support for medical and graduate students studying Histology and Pathology. Student evaluated links are ...
International Symposium on Comparative Pathology: An annual event under the auspices of the European Society of Pathology and the Academy of Medical Sciences of Croatia. Details of the next meeting and photographs of previous ones.
Museum of Human Disease: The Museum of Human Disease is located within the Department of Pathology in the Faculty of Medicine at the University of New South Wales in Sydney, Australia. The Museum contains over 2,700 specimens of diseased human tissue. Selected images of these specimens have been hotspotted to allow inter...
Nephropathology Biopsy Cases: Each includes a clinical history, photomicrographs, pathologic diagnosis, and discussion.
Online Interactive Pathology Laboratory: Offers an interactive, case-based approach to learning.
PathMax: PathMax is the premiere pathologist-created WWW metaindex of sites pertinent to pathology education. PathMax is indexed by topic and constantly updated and reviewed by pathology professionals.
Pathology jobs: Pathology jobs are listed at Physician Employment. You may register to be automatically updated when new jobs are listed.
Pathology Jobs: Physician jobs for all specialties throughout North America.
Pathology Quiz Online: Presents a series of illustrated case histories.
Pathologynet: An interactive information center about pathology and medical laboratory. Provides pathology news, numerous links (including employment, conference & industry), forum, polls, quiz and a career guide.
PathWeb: the Virtual Pathology Museum, Department of Pathology, University of Connecticut Health Center
The Internet Pathology Laboratory: WebPath contains images, text, and tutorials for pathology education
The Pathology Guy: General topics are discussed, as well as articles on autopsies, evidence collection and other forensic issues.
Transplant Pathology Internet Services: International collaboration on standardizing criteria for assessing the histopathologic features of rejection. Provides photographic case histories and discussion.
University Pathologists: Provides cytology and tissue diagnostic services for head and neck, upper and lower respiratory tract, breast, gastrointestinal, endocrine, urologic, hematopoietic and cutaneous pathology. Find details for professionals and patients.
Urbana Atlas of Pathology: Images cover all body systems and disease processes.
Virtual Hospital : Pathology Textbooks: Virtual Hospital was a digital library of health information in pediatrics, paediatrics, and radiology for pediatric education and radiology education
Wellpath: Electroninc Learning Environmet in Pathology. Used in life-long learning, distance and conventional education in University of Oviedo
This directory is based on the Open Directory and may have been modified
